In this work, regenerated silk fibroin is reinforced with metal oxide nanoparticle such as zinc oxide, copper oxide and Titanium dioxide in formic acid solution. These metal oxide nanoparticle have outstanding properties such as electrical, magnetic, optical, thermal and catalytical activity and also used to tune the properties of nanocomposite by controlling the rheological behaviour of solution. On steady shear analysis it is observed that at lower shear rates nanocomposite solution and the pure silk solution shows rheopectic behaviour while at high shear rate it is almost constant throughout the time. Zinc oxide and Titanium dioxide having almost equal viscosities at high shear rates however, addition of copper oxide nanoparticle loosen the chain entanglement on high shear in the silk which lead to decrease in viscosity more vigorously. Oscillatory sweep has revealed that addition of copper oxide exhibit no effect on silk solution as it is independent of frequency like the pure silk solution with higher storage and loss modulus compared to pure. Titanium dioxide and zinc oxide in silk solution do not exhibit a typical polymeric solution behaviour as the loss modulus is dominated at higher frequency and storage modulus is dominated at lower frequency which is the opposite of polymeric solutions trend and also the native silk feedstock solution. Creep recovery test revealed that addition of metal oxide nanoparticle convert the viscoelastic property of silk into viscous behaviour. On performing the structure recovery test by rotational shear has observed that Copper oxide gives maximum structure recovery from low-high-low shear rate in pure solution as compared to TiO2 and ZnO.