Natural biopolymers such as polysaccharides and proteins have long been used for biomedical applications such as tissue engineering, drug delivery applications.1 Hydrogels have emerged as new fabrication technique based on their ability to absorb wound exudates, similar strength as body tissues, controlled release of drugs, etc.2 Injectable hydrogels have aroused interest of the scientific community worldwide due to its ease of application as they are minimally invasive, can be administered at difficult sites.1 In this work we have compared three hybrid injectable hydrogel systems viz. alginate/gelatin (1:1), alginate/chitosan (1:1) and alginate/albumin (1:1). An efficient strategy is employed for the preparation of injectable hydrogels using the 1-Ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC) /N-hydroxysuccinimide (NHS) crosslinking followed by ionic crosslinking. The hybrid hydrogels were characterized on the basis of chemical structure (FTIR/XRD), morphology (SEM), thermal properties (TGA/DSC), swelling behaviour, mechanical properties (compression/tensile), rheological properties. Preliminary data shows the possibility of use of all the materials for injectable hydrogels for drug delivery applications. Keywords: hydrogels; injectable; crosslinking Acknowledgment: This work was supported by Ministry of Education, Youth and Sports of the Czech Republic- Program NPU I (LO1504) and Research and Development Capacities Development at TBU in Zlín – CZ.02.2.69 / 0.0 / 0.0 / 16_028 / 0006243 for mobility grant. References 1. Wang, K., Nune, K. C., & Misra, R. D. K. (2016). The functional response of alginate-gelatin-nanocrystalline cellulose injectable hydrogels toward delivery of cells and bioactive molecules. Acta biomaterialia, 36, 143-151. 2. Caló, E., & Khutoryanskiy, V. V. (2015). Biomedical applications of hydrogels: A review of patents and commercial products. European Polymer Journal, 65, 252-267.