Cellulose, one of the most abundant natural polymer, is a structural component of cell wall of green plants. Nowadays, because of not only the properties of bacterial cellulose (BC) as high biocompatibility, hydrophilicity, nontoxicity and biodegradability, but also due to disadvantages such as having low purity because of hemicellulose and lignin content which limits the preference of plant- based cellulose in industry, researchers and companies have an increasing attention on the production of bacterial cellulose (BC). On the other hand, the main drawback for the production of cellulose from bacterial strains is the productivity. Therefore, researchers have focused on finding new microorganism for the production of bacterial cellulose at high yield. Recently, a bacterial cellulose producing organism isolated from homemade vinegar was identified as Komagataeibacter sp. strain 16 by 16S rDNA sequencing analysis. Within the scope of this study, the optimization of the culture conditions and medium components to increase the productivity of bacterial cellulose were performed. According to results of this study, it was shown that, Komagataeibacter sp. strain 16 is a could have a high potential as bacterial cellulose producer for potential industrial applications.