Fused deposition modeling-based 3D printing of nylon 6 was successfully performed after the incorporation of an impact modifier and chain extender in various proportions. The analytical properties such as rheological, thermal and melt flow index (MFI) of melt extruded nylon 6-based filaments were performed to observe the suitability of such blends for 3D printing and their possible applications in biomedical applications. Melt rheology showed the improvement in storage modulus, loss modulus and complex viscosity of nylon 6 based blends after adding impact modifier and chain extender. It was also supported by MFI analysis, which showed a significant reduction in MFI value of nylon 6 after adding chain extender and impact modifier. Such a reduction in MFI was observed due to the plasticization of nylon 6, which resulted in improved 3D printability of nylon 6. Due to being a semi-crystalline polymer, nylon 6 showed various issues such as wrapping, nozzle clogging and material flowability. Such problems were fixed by adjusting various 3D printing parameters as well as by adding chain extender and impact modifier into nylon 6. A high coefficient of linear thermal expansion (CLTE) was observed in the normal direction for 3D printed nylon 6 based blend as compared with that of flow direction. This corresponds to the positioning of polymer crystals/lamella during processing. The gaps between the printed layers were observed in SEM analysis, which can be reduced further by adjusting 3D printing parameters. Acknowledgments The authors would like to thank the following for their financial support: the Ontario Ministry of Agriculture, Food and Rural Affairs (OMAFRA) – University of Guelph- Bioeconomy Industrial Uses Program Theme (Project # 030252); the Ontario Ministry of Economic Development, Job Creation and Trade ORF-RE09-078 (Project #053970) and Natural Sciences and Engineering Research Council (NSERC), Canada Discovery Grants (Project # 400320).