Fish gelatine is a biopolymer obtained from the partial hydrolysis of collagen extracted from materials such as cartilage tissue and fish scales considered as fish's industry waste. Its physicochemical characteristics and functional properties make this biopolymer of interest in different areas and one way to take advantage of it is to obtain biofilm-like materials and incorporate new molecules that modify their functional properties. In this work, fish gelatine biofilms have been obtained by incorporating glycerol plasticizer and silver nanoparticles (NAg) with the aim of modifying the functionality of the biofilms. Therefore, rheological characterisation has been carried out by determining the dynamic properties of the solutions and measurements of the elastic modulus and glass transition temperature (Tg) of their respective biofilms have been obtained. Biofilms were formed by as-cast and dried at 35 °C from forming solutions (SF) including 20% w/v fish gelatine, glycerol and NAg. NAg was synthesised using gallic acid and a reduction method, and upon UV/VIS characterisation showed a size distribution of 25 nm. [1] The SFs were obtained at 3 different NAg concentration levels, when measuring the dynamic properties of the SFs at 25 and 40 °C, a decrease in the elastic modulus (G’) and viscous modulus (G”) was observed when incorporating NAg. The solutions at 3 different levels of silver content and temperatures showed a typical liquid response (G”>G’) in the angular frequency range from 1 to 100 rad/s. Thermomechanical analysis of the biofilms showed a decrease in Tg with the addition of NAg, which could be related to a decrease in the molecular mobility of the system and the elastic modulus was modified by the incorporation of NAg, generating materials with greater elasticity, modifying part of the functionality of the fish gelatine biofilms. [1] Martínez-Castañón, N. et al., J of Nanopart Res, 2008.