Layer-by-layer (LbL) self-assembly of polymers is of interest to functionalize the surface of biomaterials. Besides, multilayers produced by LbL self-assembly technique can also be used as platforms for loading of biological molecules. These functional molecules can be released from multilayers in a stimuli-responsive manner. The physicochemical properties of LbL films are mainly determined by the chemical nature of polymers that are used as building blocks for film-assembly. In this talk, preparation of LbL films exhibiting bacterial anti-adhesive, antibacterial agent releasing and protein repellant properties will be presented. Specifically, the effect of chemical structure of polymers on biological properties of multilayers will be demonstrated through examples of multilayers with varying compositions. Moreover, surface morphology, stability, wettability and stimuli-responsive drug release properties of the films will be correlated with antiadhesive and antibacterial properties of the surfaces. Considering the ease of application of layer-by-layer technique together with the biological functionality of the multilayers, such ultra-thin polymer films are promising for modification of biomaterials. Acknowledgement: I.E.G. acknowledges the financial support of Center of Excellence in Biomaterials and Tissue Engineering, Middle East Technical University for the conference.