Nosocomial infections related to methicillin resistant Staphylococcus aureus (S.aureus) cause significant medical and economic losses in healthcare settings. In the prevention of these infections, the development of safe, non-toxic antibacterial coatings which can be applied to operating rooms, walls, medical materials and similar surfaces in health institutions is of great importance. Lysostaphin is an enzyme which is synthesized as a result of the defense mechanism of some bacteria known for their high antibacterial activity against S.aureus species, showing activity by breaking down cell walls of bacteria. In this presentation, we will demonstrate the incorporation of lysostaphin into waterborne polyurethane matrix to obtain antibacterial/antibiofilm coatings. Antibacterial coatings were prepared by immobilizing the lysostaphin enzyme onto the surface of halloysite clay nanoparticles and by integrating these nanoparticles into waterborne polyurethane coating materials. Halloysite nanotubes (HNTs) which are environmentally friendly, low cost, nontoxic natural clay nanoparticles with hollow tubular structures formed by aluminosilicate layers served as a suitable scaffold for the covalent immobilization of lysostaphin to obtain HNT/lysostaphin nanohybrids that present antibacterial activity on S. aureus. HNT/lysostaphin nanohybrids were blended into waterborne polyurethane dispersion in various proportions ex-situ and coatings were formed through spin coating. Mechanical, thermal and antibacterial/antibiofilm properties of nanocomposite coatings will be presented.