Because of biocompatibility and biodegradation of polylacticacid, it is one of the most important polymers in medical industry. , but However, it is brittle nature characteristics has restricted its usage in manyvarious applications. The main goal of this paper is utilization of soybean oil as toughener phase and Nanoclay particles to improve impact resistance of mentioned nanocomposite. To achieve this goal, nanocomposite samples with different percent of soybean oil and Nanoclay was fabricated by melt processing. To investigate the morphology and microstructure of prepared samples, Rheometry, Scanning electron microscopy, Dynamic Mechanical Thermal Analysis ,Differential Scanning Calorimetry, and mechanical tests were performed. The results show that soybean oil and poly-lactic acid are partly compatible, but in high soybean oil percentage, obtained alloys has two phases and matrix-dispersion morphology. Furthermore, it has been observed that Nanoclay particles have high thermodynamic tendency to soybean oil. In order to capsulate a larger portion of nanoparticles in soybean oil droplets, they were mixed using a mechanical mixer at temperature about 60°C. Then the obtained mixture of Nanoclay and soybean oil was mixed with poly lactic acid. Mechanical tests showed that fabricated nanocomposites