Recently, mankind has become dependent on petroleum based non-biodegradable materials that affect the ecological system negatively. Poly(lactic acid) (PLA) is an important polymer on the agenda because of its renewable and biocompatible nature. Shape memory polymers (SMP), new class of stimuli responsive materials, have shown a fast response in changing their temporary shapes to original shapes when subjected to a certain stimulus, such as temperature, light, electric field, pH, etc. PLA, as a polyester, exhibits shape memory behavior when programmed. In order to be used in physiological conditions, PLA is not suitable since its shape transition temperature is relatively high. The blending of PLA with other biocompatible polymers is a quiet simple and an economical method to optimize its properties. Thermoplastic polyurethanes (TPUs) are one of the mostly used blend components in PLA based compounds and it’s one of the most preferred SMPs. In this study, it is aimed to develop a layered composite SMPs targeted to be used in-vivo conditions for the first time in literature by using PLA/TPU. Particularly in the current study, the shape memory properties under different loads were focused with respect to number of layers, layer construction and temperature. Moreover, the toxicity and the biocompatibility of the layered composites were examined. This study is granted by KOU BAP (Grant Number: 2018/097)