In recent years, as people’s health consciousness increases, hygiene concerns regarding industrial products has drawn increased attention to antimicrobial materials. While chemical antimicrobial agents available in industry is declared as hazardous with respect to environmental and human applications, natural antimicrobial agents like essential oils (EOs) have been of interest due to their safer natural structure. Among EOs, thyme, sage and rosemary have shown antimicrobial activity against a wide variety of microorganisms. Thermoplastic elastomers (TPEs) contain a biphasic structure resulting from the combination of the elastic properties of traditional thermoset rubbers and the processing characteristics of the thermoplastic polymers. Styrene-(ethylene-butylene)-styrene (SEBS) based TPEs are the most widely used in a variety of applications including biomedical materials, household appliances, toothbrush, cables, toys, footwear etc. The aim of this study was to produce novel EOs loaded SEBS-based TPEs and to investigate their antimicrobial, mechanical and physical properties. Thyme and sage EOs were included as antimicrobial additives during the production of the TPEs. Control samples without EOs addition were also prepared. Antimicrobial properties, mechanical strength, hardness and abrasion resistance tests were performed to characterize all the samples produced. While thyme EO-loaded SEBS based TPE exhibited antimicrobial activity, sage EO-loaded SEBS based TPE showed lack of antimicrobial activity. The results revealed that the EOs loading does not significantly affect the mechanical and physical properties of SEBS-based TPEs. It can be concluded that the use of thyme EO as a natural antimicrobial agent in SEBS based TPEs is promising and has a great potential particularly for biomedical products where hygiene standards are crucial. Keywords: Antimicrobial, Essential Oil, Thyme, Sage, Styrene-[ethylene-butylene]-styrene, Thermoplastic Elastomer Acknowledgments; This work was supported by Arcelik A.S Central R&D.