Three-dimensional printing (3DP) is one of the rapidly growing additive manufacturing technologies due to its ability to print biocompatible polymeric scaffolds directly. Fused Deposition Modelling (FDM), one of the many 3D printing methods, uses specialized printers in which the extruded material is deposited, layer after layer, in the desired shape. This method for scaffold fabrication is widely applied to various type of scaffold fabrication due to the precise control it offers over the scaffold architecture at the micron-level. In the past, a number of 3D scaffold designs have been fabricated to explore the mechanical and biological viability of a 3D printed Polylactic acid (PLA) scaffold as a construct which could support cellular life. However, all AM approaches require a computer-aided design (CAD) file, from which the complex shapes are read via a 3D scanner. Moreover, the commercial 3D printer is expensive as compared to the 3D pens. This study aims to compare the mechanical properties of the scaffolds fabricated by using a 3D printer and a 3D pen. A 3D pen can perform the same functions as that of a 3D printer, with the advantage being that no CAD files are required. 3D pens are cheaper than 3D printers and lie in the price range of 15 USD to 150 USD. They allow for higher flexibility and creativity to suit the desired application and consume lesser time for fabrication. Compression testing was conducted on the 3D printed scaffolds which were printed using a 3D printer and 3D pen. Fourier Transform Infrared Spectroscopy (FTIR) was utilized to characterize both the bulk and surface chemistry of the samples. Scanning Electron Microscopy (SEM) and Differential Scanning Calorimetry (DSC) were used to characterize morphology, crystallinity and the glass transition temperature following printing. Key differences between the samples in terms of compressive strength were found and reported. Keywords: Fused deposition modeling (FDM), 3D printing