Abstract Thermo-responsive nanofibers have been highly considered in many applications more importantly in biomedical fields such as tissue engineering, drug delivery, wound dressing and etc. The polymer chains undergo conformational changes in the water as the temperature reaches the lower critical solution temperature (LCST) or the upper critical solution temperature (LCST). Poly(N-vinylcaprolactam)-based nanofibers have gained interests recently in biomedical applications on the grounds that their chains conformation change at almost near body temperatures. Poly(N-vinylcaprolactam)-b-poly(vinyl actetate) thermo-responsive polymer chains partly interact with water below the LCST and by heating the copolymer above the LCST, hydrophobic intermolecular interactions overcome the water-polymer interactions through conformational changes. This copolymer also shows no considerable cell toxicity. The aim of this study is to fabricate PNVCL-b-PVAc electrospun nanofibers suitable for variety of applications such as wound dressing and drug delivery. The mechanical properties, water uptake, morphology, porosity, contact angle and shrinkage behavior of fabricated non-woven nanofiber mats would be evaluated.