In the conventional imprinting process, the substrate and the stamp are brought into contact and compressed directly by the plate and rigid mold. This Air pressing process provides uniform embossing pressure with UV curing enables the process to be performed at room temperature and low pressure. The UV resin is sensitive to the process parameter such as the curing time and the pressing pressure. In order to precisely control the process, we developed an Automatic UV-curing imprinting machine, which contains glue spreading system, Air pressing system and UV curing system. All system were controlled by the Arduino interface. With Arduino system, all forming step could be programed into one script to eliminate artificial error. In this study, innovative mechanisms with UV curing and air pressing imprinting were proposed. The V-groove microstructures were employed as the pattern. The soft mold pressure only effect the residual layer thickness but with very little effect on the replication of microstructure. The resin filling could be achieved in a tiny pressure given because the resin is in liquid state. We had successfully replicated V-groove pattern with 47 um width 23 height on P Poly methyl methacrylate (PMMA) substrate which could be used as light guide panel. Key words: Microstructure; Light guide plate; UV imprint lithography; Air pressing.