Components with surface nanostructures for biomimetic dry-adhesion has a great potential in many application such as gecko tape, climbing robot and skin patch. In this study, Nanosphere lithography with self-assembly of nanospheres was developed to achieve effective and efficient fabricating of dry-adhesion structure. Self-assembled monolayer nanospheres with high regularity can be obtained by tilted dip coating. The RIE etching of the self-assembled nanosphere can fabricate nanostructures of different shape and aspect-ratio by varying the etching time. Then nickel molds with inversed nanostructure were replicated by the electroforming process. By gas-assisted hot embossing method, the PDMS nanostructures were fabricated. The pulling test was performed to measure the shear adhesion on glass substrate of the sample. The static contact angle measurement was used to verify the hydrophobic property of structure. The results show that structure can enhance the adhesion force to 4.05 N/cm2 and the contact angle on the PDMS with nanostructure is 135.2°. This columnar structure can effectively enhance the adhesion ability of PDMS, demonstrating the potential of using the nanosphere lithography process for fabrication of adhesion.