We continuously fabricate nanofeatured anti-reflective films, for the performance enhancement of solar cells, using hot roller imprinting and self-assembly nanosphere-patterned, nickel-cobalt membranes. Polystyrene nanospheres of different sizes (471, 628, and 1200 nm) were first self-assembled onto Si substrates by a spin coater. A thin layer of aluminum was then deposited onto the surface of nanosphere-patterned substrates, using the plasma sputtering technique. After electroforming, nickel-cobalt membranes containing nano-array of different sizes were obtained. The membrane was then attached to the surface of the metallic roller in a hot roller imprinting facility. The imprinting facility was used to copy the nanofeatures onto 60 um thick polyethylene terephthalate (PET) films in the experiments. The imprinted films were characterized using water contact angle measurement, a UV–vis spectrophotometer, atomic force microscope (AFM), and scanning electron microscope (SEM); while the conversion efficiency of the films for the solar cells was also measured by a solar simulator. The measured results suggested that the imprinted films could effectively reduce the reflectance and increase the conversion efficiency of solar cells. The proposed method shows great potential for fast fabrication of the anti-reflective, protective layer of solar cells, due to its simplicity and versatility.