The shear-induced isotactic polypropylene (iPP) trancrystallinity (TC) results in two crystal form, known as α-cylindrites and β-cylindrites. In this study, the trancrystallization of iPP induced by carbon fibers pulling under gradient temperature field (g-T field) was systematically investigated. A combined effect of temperature and shear rate on the formation of β-iPP cylindrites can be identified by stepwise adjust pulling rate under continuous temperature field. The results demonstrated that the trancrystallization depended strongly on the both carbon fiber pulling rate and the temperatures. It was found that the β-iPP cylindrites can only be produced under the condition of fiber pulling rate higher than 100 μm/s. Moreover, the trancrystallization varied from α-iPP cylindrites to mixed α-iPP and β-iPP cylindrites and finally pure β-iPP cylindrites along the g-T field from 150 °C to 130 °C. It was demonstrated that there is a highest temperature of 145 °C for the formation of β-iPP cylindrites, which is indenpendent on the pulling rate. Such temperature (145 °C) is highly possible as a starting temperature for ocurrance of β-to-α transition. So the appearance of β polymorph in iPP TC is essentially dominated by the thermodynamic stability of β-form. The findings in our study are meaningful for the manipulation of polymorphic structure of iPP trancrystallization.