Fabrication of small diameter vascular scaffolds has been a challenge in recent years, especially scaffolds with multiple layers. In this study, two approaches were proposed to fabricate multiple-layered vascular scaffolds by hybrid electrospinning, braiding, and thermally induced phase separation methods. It was found that the electrospun fibers had a compact fibrous structure that provided good mechanical properties. The porous TIPS layer had high porosity and pore interconnectivity to facilitate cell penetration; however, this structure alone could not ensure sufficient mechanical strength for surgical applications. Double and triple-layered vascular scaffolds consist of different layer materials with various structures were fabricated by applying different methods alternatively. The triple layered scaffolds comprised of nanofibrous inner layer, woven silk filament middle layer, and porous outer layer were found can mimic the structure of native blood vessels. They have a desirable toe region in tensile tests and sufficient suture retention and burst pressure for vascular graft applications. Endothelial cell culture tests showed that a cell layer could form on the inner surface of the scaffold with high cell viability and favorable morphology. Keywords: Multiple-layered vascular scaffolds, electrospinning, thermally induced phase separation, mechanical properties, human umbilical vein endothelial cells