Electrospinning of natural and synthetic functional polymers is a common technique used to generate aligned fibers of varying diameters, and the potential use of these fibers in tissue engineering has been studied extensively. However, the ability to control the fiber microarchitecture and thereby important biological functions, such as spatially controlled presentation of biomolecules, is a key challenge in regard to successful tissue regeneration. In this communication, we demonstrate a novel type of micro structured scaffold material comprising multicompartmental microfibers. These scaffolds are prepared by electrohydrodynamic co-spinning of two or more poly (lactide-co-glycolide) (PLGA) polymers, which yields biodegradable microfibers with multiple, chemically distinct compartments.