Electrospinning is an efficient, convenient and low-cost technology for preparation of fibrous materials with very fine diameters ranging from submicron to several nanometers using a high voltage power supply. It has the ability to produce fibers that are far smaller than those produced by conventional means of fiber spinning, such as wet spinning, dry spinning, melt spinning, and gel spinning. Chitosan (CS), a (1→4)-linked 2-amino-2-deoxy-D-glucopyranose, is a polysaccharide which is the N-deacetylated derivative of chitin, although this N-deacetylation is almost never complete. Chitosan is partial chemical deacetylation of chitin, the second most abundant biopolymer after cellulose in the world, which is extracted from crustaceans by deproteination, decalcification and decolorization and from fungi by enzymatic extraction. The mica group of sheet silicate (phyllosilicate) minerals includes several closely related materials having close to perfect basal cleavage. However, only a few research reports on CS/mica nanofiber mats are available, leaving its potential suitability for many applications, including as a wound dressing material. In this paper, we research the parameters of producing chitosan/PEO/mica fiber. The surface morphology and mechanical properties of the fiber was studied by using SEM, AFM, UTM, TGA, DSC. We also compared the antibacterial properties of chitosan/PEO and chitosan/PEO/mica nanofiber mats.