The synthesis of nanoparticles from plant extract through eco-friendly green chemistry has recently drawn attention of researchers with a view to develop TiO2 nanoparticles. Many plant extracts like Lemon Grass, Indian Gross berry and Neem Leaves, have been tried for the synthesis of TiO2 nanoparticles. In the present, we reported the synthesis of TiO2 Nano particles by using extracts of Neem Leaves and Titanium isopropoxide. The characteristic properties of TiO2 nanoparticles were studied by XRD, SEM/EDX, TEM, UV-VIS and FTIR analytical techniques to confirm their structural, morphological and optical attributes. The sharp peak of XRD pattern exhibit the polycrystalline nature and formation of TiO2 rutile structured nanoparticles. SEM/EDX and TEM images revealed the spherical structure of nanoparticles with size varying in the range of 10-20 nm. UV-VIS spectrum recorded in 1100 – 200 nm region showed the excitonic emission peak at abour 380 nm while IR transmission spectrum showed the characteristic stretching modes of TiO2. These results confirmed the synthesis of TiO2 nanoparticles via non-hazardous green chemistry route by using neem leaves extract. Keyword – TiO2 Nano particles, Neem (Azadirachta Indica) Extracts, Titanium Isopropoxide, Green Chemistry