Magnetic nanofibers (MNFs) are a various array of Electro Active Polymers, which has transmitting response to an electrical magnetic field. This kind of polymers has several applications such as artificial muscles, wave absorbers, electrical insulators and etc. MNFs usually consist of polymeric nanofiber as a substrate and magnetic nanoparticles like iron, cobalt and etc. There are various methods for nanofibers production but Electrospinning process prefers to other methods in terms of speed, diversity, simplicity, cost, and the possibility of commercialization. Furthermore Aluminum doped Iron oxide nanoparticles also have high chemical stability and oxidation resistivity comparing to pure iron oxide nanoparticles. In this research, Polyacrylonitrile (PAN) had been electrospun with and without magnetic nanoparticles (Aluminum doped γ-Fe2O3, 80nm mean particle size) and turned into nanofibers. To make the mentioned nanofibers, firstly 15 wt% of PAN was solved in dimethylformamide (DMF). After that the nanoparticles were added to the solution with two distinct weight percentages of PAN (4wt% and 8wt%) and then were electospun after sonication. During the electrospinning process because of high voltage, DMF was evaporated. The effect of nanoparticle content on magnetization of PAN nanofibers was investigated. The results have shown that the diameter of the nanofibers decreased from 700 nm to 300 nm with addition of the magnetic nanoparticles. Meanwhile, the pure PAN nanofibers have low magnetic ability due to polar bonds that exist in acrylonitrile groups. With addition of only 4wt% of the magnetic nanoparticles to PAN nanofiber, it can be clearly seen that magnetization ability soared more than 10 times but at higher percentage, it showed just a little change on the grounds that a high negative interaction between nanoparticles. As suggested in other works, nanoparticles are antiferromagnetically coupled due to incomplete dispersion at higher compositions.