By the combination of top-down and bottom-up methods, robust coatable polarizing thin film was successfully fabricated by the self-assembly of lyotropic chromonic liquid crystals and the subsequent photo-polymerization. Their molecular packing structures and optical behaviors were also investigated by the combined techniques of microscopy, scattering and spectroscopy. To stabilize the oriented lyotropic chromonic liquid crystal films and to minimize the possible defects generated during and after the coating, the photo-polymerization of acrylic acid (AA) monomers added to the solution was carried out. The polymer-stabilized lyotropic chromonic liquid crystal films showed good mechanical and chemical stabilities with maintaining a high polarizability. Additionally, patterned polarizers were fabricated by applying a photo-mask during the photo-polymerization of AA, which may open new doors for the practical applications in electro-optic devices.