Inspired from the nacreous structure, here we precisely designed the high-performance zinc oxide (ZnO) whiskers reinforced poly(lactic acid) (PLA) composites during the injection molding. Two important features involved in our approach that distinguished it from the others were the application of an intense shear flow and the carefully selected whiskers that showed a wide range of diameter from several nanometer to 1-2 orders of magnitude higher. In the shear flow, whiskers were impelled to regularly align along the flow direction, while PLA chains were sufficiently stretched and oriented to form the row-nuclei or shish. Multiscale assemblies of superstructures were observed. Specifically, the strong interest stemmed from the simultaneous formation of multiple kebab consisting of the central hybrid shish-kebab induced by the nanowhisker and a transcrystalline layer, transcrystallinity developed at the surfaces of micro-sized whiskers, and classic shish-kebab and multiple shish structures in the bulk. The multiple kebab and multiple shish structures, which were both first observed for PLA, were presumably due to the anchoring interactions of nanowhiskers and shear stress gradient, and the stretch of entangled chain networks. Such a composite system presented perfect UV shielding efficiency and impressive resistance to heat distortion, as well as unprecedented combination of mechanical properties reflecting by the tensile strength of near 120 MPa, Young’s modulus of 2.3 GPa, elongation at break of 8.7% and impact strength of 11.5 KJ/m2. The biomimetric origins were responsible for the unusual combination of strength, stiffness and toughness. In fact, the well aligned miscro-sized whiskers and nanowhiskers were prone to dissipate and absorb much energy before the catastrophic failure and showed strong retardation of crack propagation from layer to layer, while the crystalline superstructures decorated at whisker surfaces acted as the ligament or filament formation in the interfaces.