Urinary Tract Infection (UTI) is a common problem in the population and can affect from neonatal to the elderly, both men and women. 35% to 45 % of all hospital-acquired infections is estimated to be ITU. Late diagnosis of UTI increases the risk of kidney injury; it may result to serious sequelae such as renal failure and hypertension. In pregnant women, the delay in diagnosis of UTI can lead to complications, such as labor and pre mature birth, premature rupture of membranes and even the restricted growth in utero. Complications during pregnancy because of UTI are associated with fetal mortality observed twice in the same geographic area. Although rare, it has been found cases of excess mortality in patients who acquired UTI within 72 hours after the hospitalization period up to three times (Nosocomial UTI). Clearly the need to create ways of diagnosing UTI fast , simple and reliable application to accelerate the treatment and cure trying to reduce and even eliminate the occurrence of complications to the health of individuals suffering from this problem. This study developed and evaluated a sensor system based on conductive polymers (polyaniline) for the identification of Ammonia and nitrate, measurement of pH of human urine samples and developed an electronic circuit using the Arduino platform for interfacing with sensor ITU.