Currently lignin is found readily as a byproduct in the pulp and paper industry and bioethanol production plants but lacks the demand for the large quantities being produced. Scientists are now looking into ways in which lignin can be used to replace petroleum-based carbon in various applications. This work outlines the use of bioethanol based lignin as a substitute for carbon black by carbonization and ball milling optimization processes. The microstructure, surface area, particle size and resistivity were characterized against a commercially available carbon black to determine the viability of the carbon powder produced. Acknowledgements: This research is financially supported by the Ontario Ministry of Economic Development and Innovation (MEDI), Ontario Research Fund - Research Excellence Round 4 program & Ontario Ministry of Agriculture, Food, and Rural Affairs (OMAFRA) New Directions and Alternative Renewable Fuels research program.