Polymer scaffolds are applied in the field of tissue engineering to direct generation of a damaged tissue.In situe gelling scaffolds have attracted great attentions, as they are structurally similar to the extra cellular matrix (ECM). However, their potential for tissue reconstruction depends on both biological behavior and mechanical characteristics in microscopic and microscopic levels. The goal of the present work is to design and prepare a new injectable and crosslinkable hydrogel based on partially hydrolyzed polyacrylamide (PHPAM) and chromium acetate for the regeneration of cartilage. The distinct characteristic of PHPAM is the presence of carboxylate anion groups on its backbone which allows to engineer the structure of the hydrogel for the desired bioactivity with appropriate cells differentiation towards both soft and hard (bone) tissues. The synthesized hydrogel exhibited bifunctional behavior which was derived by its biphasic structure in which one phase was loaded with nano hydroxyapatite to provide integration capability by subchondral bones, and the other phase differentiates the rabbit adipogenic mesenchymal cells towards soft tissue. Rheometrical spectrometry (RMS) was employed to study the kinetic of the gelation including induction time and rate, as well as to measure the ultimate elastic modulus of the optimum crosslinked hydrogel. Atomic force microscopy (AFM) and surface tension measurement were also performed to tailor the surface characteristics of the gels. In vitro culturing of the cells inside the crosslinked hydrogel revealed high viability and high differentiation of the encapsulated rabbit stem cells, providing that the chromium acetate level was kept below 0.3 wt%. In addition, the time dependency of the gels towards micromechanical signals showed to influence the cells attachment and differentiation. Based on the obtained results, the designed and fabricated biphasic hydrogel exhibited high potential as carrier for the stem cells for cartilage tissue engineering application with excellent injectability. Keywords: Hydrogel,Nanocomposite,Injectable,Partially Hydrolyzed Polyacrylamide,Chromium Acetate,Nano Hydroxyapatite