The influence of micro- and nanostructures on the wetting behavior of surfaces is widely debated. This work examines four different polymers and their change of wetting behavior due to structures on their surfaces. Polymethylmethacrylat (PMMA), Polysulfon (PSU), Polycarbonate (PC) and Cyclic Olefin Copolymer (COC) were used in the experiments because of their wide use in biological and medical applications. The thermoplastic polymer foils were structured by hot embossing, a high-precision manufacturing process. Hot embossing is used for the structuring of polymer foils. A polymer foil is put between a mold insert and a substrate plate and is heated over the glass transition temperature. After reaching the molding temperature the mold insert with the negative structures is isothermally pressed into the foil. The pressure is kept constant during cooling until the demolding temperature is reached. The system is then opened and the foil is demolded. In our work structures with diameters down to 250 nm and a height of 300 nmwere successfully replicated. Dylindrical micro- and nanopillars with diameters of 250 nm, 500 nm, 750 nm and 1 µm were fabricated. The distance between the structures was n*diameter for n=1..4. The structured foils were examined by contact angle measurements with a Dataphysics OC30 instrument. Static contact angle measurements were conducted. A water droplet was put on the surface and the angle on the triple point between the droplet and the surface was measured. In our work we could show the dependency of the contact angle from pitch and diameter of the surface structures. While the increase of contact angle due to the structures is independent of the material, the absolute value of the contact angle is dependent on the unstructured materials contact angle. With this information the fabrication of microfluidic systems with tunable wetting behavior is possible.