Since their discovery, nanoparticles are changing many basic concepts of science and they have received undivided attention because of their fundamental unique physical and chemical properties. Nanoparticles are known to exhibit remarkable properties such as high surface area, chemical stability, electrical conductance and electrochemical activity at nanoscale in electrochemical sensors application. Therefore the use of various nanoparticles in fabrication of glucose sensor have gained considerable attention due to some specific drawbacks of the enzymatic glucose sensor such as complex enzyme immobilization, thermal and chemical instability, critical operating conditions such as optimum temperature and pH. Thus, for the development of non-enzymatic sensor based on nanoparticles have offered a simple, cost effective, high sensitive and direct approach compared enzyme based sensors. In this work we report on electrochemical characterization of copper nanoparticles on in house produced screen printed electrodes (SPEs). The conductive properties were investigated electrochemically making use of a 1mM ([Fe(CN)6]3−/4−) redox probe. The particles were further tested for Electrocatalysis and electroanalysis of glucose by modification of in-house produced carbon screen printed electrodes (NICsens).