The polyamide thin film composite (TFC) membranes have been intensively employed in a reverse osmosis process for seawater desalination owing to their excellent salt-rejection and stability over a wide range of pH and temperature operations. However, polyamide based membranes still suffer severe biofouling and chlorine attack, which lead to increased energy consumption, decreased membrane performance, short life-span and ultimately high operational costs. Therefore, the design of anti-biofouling and chlorine-tolerant membranes without compromising productivity and selectivity is of great importance to date. Chemically modified-graphene oxide possesses great potential for application in water treatment owing to its excellent physiochemical properties; ultra-fast water transport, chlorine-resistance, anti-biofouling properties and compatibility in polymeric matrices. In this study, we report on the imidazolidinyl urea-functionalized graphene oxide (IU-GO) synthesized by covalent grafting of imidazolidinyl urea onto graphene oxide. Subsequently, a novel polyamide thin-film composite membrane embedded with IU-GO was developed. The as-synthesized membrane showed enhanced anti-biofouling and chlorine resistance with very minimal effect on the flux and salt rejection. The results acquired from FTIR, Raman spectroscopy, AFM, SEM, TEM, and XRD will be presented. The influence of the chemically-modified graphene oxide loading on the hydrophilicity, surface charge, surface roughness, and thickness were also evaluated.