We herein report a thiomalic acid capped (TMA) capped CuS nanoparticles incorporated polyaniline (PANI) composite based sensor for the electrochemical detection of D-Glucose. The unique thiomalic acid (TMA) capped copper sulphide (CuS) nanoparticles were synthesised using a one-pot chemical growth method at 95 °C. The TEM analysis showed that the produced TMA-Cu2S nanoparticles had an average particle size (APS) diameter of 5.4 nm. The CuS nanoparticles were highly crystalline with the dominance of djurleite C31S16 phase, confirmed by X-Ray Diffraction (XRD) spectroscopy studies. The TMA capping on the CuS nanoparticles showed improved solubility due to the presence of the carboxyl (-COOH) functional groups present in one of its end terminuses. The presence of the TMA capping on the surface of the CuS nanoparticles –COOH functional group thereof was confirmed through Fourier infrared (FTIR) spectroscopy studies by the occurrence of aliphatic –C=O, -C-O and -OH bands at 1638 cm-1, 2083 cm-1 and 3311 cm-1 respectively. Incorporating the TMA-CuS onto the PANI exhibited a platform with improved electrochemical activity, improved electron transport and faster electron transfer kinetics. The proposed alignment or arrangement on the screen-printed carbon electrode (SPCE) surface takes the advantage of the excellent electron conductivity, good adhesion properties, remarkable affinity and adsorption properties of the PANI, which in turn allowed for further improved electroactivity exhibited by the platform during the detection of D-glucose. The electro-oxidation of D-glucose on the PANI/CuS-TMA composite occurred at the formal potential (Eoˊ) of 0.26 V. The SPCE/[PANI/TMA-CuS] platform was capable of detecting the D-Glucose with an excellent dynamic linear range (DLR) from 6 mM to 16 mM and low limit of detection (LOD) of 5 mM.