Agave tequilana Weber var. azul (AT) fructans are fructose-based polymers that constitute the main raw material for the production of tequila. Chemically, they are β-(2,1) and/or β-(2,6)-linked fructose polymers anchored to a glucose residue found in the vacuoles of many plants including the Agavaceae family [1, 2]. In conventional tequila production, only AT “heads” are used, whereas the leaves are considered useless; regardless their high total reducing sugars (RS) content (up to 16.1%) [3]. Green synthesis of silver nanoparticles (AgNPs) encompasses the use of plants extracts containing natural reducing compounds (e.g. RS) for an eco-friendly and economically sustainable production of AgNPs [4]. In the present work, we demonstrate the use of an extract obtained from a major Mexican agroindustrial residue (i.e. AT leaves) for the synthesis of AgNPs. Additionally, the antimicrobial activity of the green-synthesized AgNPs was evaluated against human pathogenic bacteria. Results indicated that AT-extract could be used as a reducing agent to obtain AgNPs as those shown on Fig 1a. The as-synthesized AgNPs significantly inhibited bacterial growth against Staphylococcus aureus, Pseudomonas aeruginosa and Escherichia coli as exemplified by the inhibition zones presented on Fig 1b. 1. Arrizon, J., et al., Comparison of the water -soluble carbohydrate composition and fructan structures of Agave tequilana plants of different ages. Food Chem, 2010. 122: p. 123-130. 2. Narváez-Flores, M., et al., Ultrasound assisted extraction modeling from agave (Agave tequilana Weber var. Azul) at different temperatures and ultrasound powers. Food Bioprod Process, 2015. 96: p. 232-239. 3. Iñiguez-Covarrubias, G., et al., Utilization of by-products from the tequilana industry. Part 2: potential value of Agave tequilana Weber azul leaves. Biores Tech, 2001. 77: p. 101-108. 4. Meshram, S.M., et al., Green synthesis of silver nanoparticles using white sugar. IET Nanobiotechnol, 2012: p. 1-5. Fig 1. (a) SEM micrograph of green synthesized AgNPs Agave tequilana Weber cultivar azul extract, (b) bacterial growth inhibition zones displayed by AgNPs against Escherichia coli