This study seeks to develop hydrogel formulations for injectable controlled drug delivery from Pluronics incorporating multiple drugs, enhancing patient compliance, decreasing side effects, reducing dose and frequency. Pharmaceutically, these systems are attractive due to their unique sol-gel phase transition in the body, biocompatibility and safety being injected into the body as solutions before transforming to gel matrices at body temperature without external stimulation. Pluronic F-127 is a block copolymer (PEO-PPO-PEO) that gels at low concentrations and can be tuned using other excipients. Real-time full structural studies of F-127 systems mixed with a control drug and/or different molecular weight Pluronics under controlled temperature gradients and shear were performed. New experiments combining multiple characterisation techniques including rheology, X-ray scattering and Raman were developed to provide practical measurements of the system dynamics, essential for both application and manufacture.