Present work is based on the antimicrobial and antistatic performance of conducting polymer nanocomposite impregnated fabric based on polypyrrole (PPY) and copper oxide nanoparticles (CuO NPs). Conducting polymer nanocomposites (PPY-CuO NPs) were synthesized by insitu chemical oxidative polymerization of pyrrole using sodium lauryl sulphate as a surfactant and ferric chloride as a dopant. Coating of PPY-CuO-nanocomposites on the cotton fabric was carried out during polymerization. Interaction of CuO NPs with PPY matrix was determined by the FTIR spectra, Thermo-gravimetric Analysis, X-Ray diffraction, Scanning Electron Microscopy (SEM), High Resolution Transmission Electron Microscopy (HRTEM) and conductivity measurements. The conductivity of PPY-CuO NPs coated fabric was found in the range of 10-8 to 10-3 S/cm depending on the loading concentration of CuO NPs in polymer matrix. TEM and HRTEM images showed that the PPY-CuO nanocomposites with an average diameter of 50-60 nm and were found to be nicely dispersed in the polymer matrix. The PPY-CuO NPs coated cotton fabric were tested against Escherichia coli (Gram negative) and Staphylococcus aureus (Gram positive) cultures and showed a significant antimicrobial activity; whereas its analogous uncoated and polypyrrole coated material showed almost negligible activity against the bacteria. Antistatic performance of the nanocomposite impregnated fabric was investigated by John Chubb Instrument. The static decay time of the nanocomposites coated fabric was found to be in the range 0.4 to 1.0 s on recording the decay time from 5000 V to 500 V. This indicated that the nanocomposite based on PPY-CuO nanocomposites has a great potential to be used as an effective antimicrobial and antistatic material. Moreover, the synergistic role of conducting polymer and CuO nanoparticles for antimicrobial and antistatic applications has also been studied.