Crystalllization of polyethylene usually results in elongated lamellar crystals alternating with inter lamellar amorphous layers, while the lamellae normally growth in a radical direction to form the spherulites. All these structural features contribute to the mechanical behavior that makes complex structure-property relationships. Previous study has proved that the deformation of spherulites is heterogeneous, which strongly depends on the local stress and microstructure distribution in spherulites. However, due to its complex microstructure, it is difficult to measure the local stress in spherulites. The infuence of the microstructure on the local stress distribution of spherulites in small deformation region has been studied by using a set of polyethylene samples with different microstructures. The local stress in the polar and equatorial rgions of spherulite were estimated via in-situ wide angle scattering (WAXS) experiments which permits to get a correlation between local and macroscopic stress. Corelating the local stress with microstructures, three main results were obtained: (1) a linear relation exists between macroscopic stress and local stress in polar and equatorial regions of spherulites in pre-yield domain; (2) local stress in polar region (σ_local^polar) is higher than the local stress in equatorial region (σ_local^equ), while the ratio of σ_local^polar/σ_local^equ increases with increasing crystallinity; (3) percolation of crystalline lamella and the stress transmitter density of interlamellar amorphous are the two major factors determing the local stress distribution. A explaination model was proposed as that: higher crystalllinity promotes well connection of crystalline network resulting in the more stress transfered from polar lamellae, while higher crystallinity also relates to the less concentration of stress transmitter leading to the weak connection between crystalline lamella and interlamellar amorphous phase which causes less stress transfere