Abstract: Joint injury is a common clinical disease. Previous study found that the damaged adult cartilage can be self-repaired if the damaged area was less than 3mm diameter, otherwise it cannot be repaired[1]. In this work, the prosthesis integration structure was designed and prepared by using the two-step molding injection method [2]. The EVA [3] was used as upper artificial cartilage layer, and HA/PA66[4]scaffold for underlying layer. The integrated EVA-HA/PA66 joint prosthesis showed good mechanical and bio-tribological properties. And, the in vitro experiment has shown that EVA and HA-PA66composite has no toxicity for cells. Furthermore, the in vivo results in this work proved that, the integrated prosthesis showed high bioactivity to induce the formation of new bone and excellent biocompatibility with the surrounding tissues. Therefore, the EVA-HA/PA66 composite prosthesis will have a wide prospect to be used in joint replacement. Key words: Joint prosthesis, EVA, HA/PA66 Acknowledgements This work was supported by the National Natural Science Foundation of China (Grant no. 51273028), and the Key Technologies Research Program of Sichuan Province (Grant no. 2014SZ0019-6). References [1] Kin KW, et al.. J Bone Joint Susr. 1991, 73(9), 1301-1315. [2] ZePu Wang, et al..JMater Chem B. 2015, 3, 75–85. [3] Nabakumar Pramanik, et al..Mater. Sci. Eng C. 2009, 29, 228-236. [4] Huanan Wang, et al..Biomaterials. 2007, 28, 3338–3348.