Polyethersulfone (PES), polyetherimide (Ultem® 1000), and polyimides (Matrimid® 5218 and P84) are common commercial polymers used to produce hollow fibre membranes for different gas separation applications. In this work, asymmetric hollow fiber membranes were prepared using these polymers by a phase inversion technique. The effect of spinning parameters (composition of the dope and bore solution, bore flow rate, air gap distance, temperature of the spinneret and coagulation bath, as well as take-up speed) on the membrane structure and gas permeation properties was investigated. The membrane separation performances were characterized by measuring their gas permeation properties (permeability and selectivity) for different gases (H2, CO2, O2, N2, and CH4) and by their cross-sectional morphology using scanning electron microscopy (SEM). The relationship between the hollow fiber gas performance and their intrinsic gas properties of dense flat membranes using the same materials were also studied. A comparison between the average apparent skin layer thickness calculated from O2 permeability/permeance, and the results based on SEM images was made, and good correlations were obtained between both results.