Surface texturing technology has been widely used to improve the functionality of materials surface wettability and frictional characteristics in recent years. This is because the micro-structured surface such as regularly microchannels can contribute to friction reduction at sliding contact interfaces and can provide micro-reservoirs to enhance lubricant retention or micro-traps to capture wear debris. For the purpose of developing the new tribomaterials, the fabrication of micro-structured surface of polyamide (PA) using femtosecond laser and their frictional properties were investigated. In this study, the effect of processing parameter such as laser power, laser speed, laser spot size, focal length and pitch distance on the fabrication of micro-structured surface of polyamide 66 (PA66) and plants-derived PA1010 were investigated experimentally, and their frictional properties and wettability were evaluated. Frictional properties of these polyamides were measured by a reciprocating type sliding wear tester at constant normal load and sliding speed under dry and lubrication conditions. Wettability of the various polyamides with surface microchannels was measured by a contact angle gauge. It was found that the surface microchannels are able to be fabricated by the femtosecond laser and have a good effect for improvement of the frictional properties and wettability of polyamides. This is attributed that the micro-structured surface has played an important role in the key components for polymeric tribomaterials.